UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL  DAUN MANGKOKAN (Polyscias Scutellaria) TERHADAP BAKTERI Bacillus cereus DAN Shigella dysentriae

Sri Hainil; Rury Trisa Utami; Delladari Mayefis; Nurul Asiqin

doi:10.59003/nhj.v5i5.1710

Authors

Sri Hainil Institut Kesehatan Mitra Bunda
Rury Trisa Utami Institut Kesehatan Mitra Bunda
Delladari Mayefis Institut Teknologi Sumatera
Nurul Asiqin Institut Kesehatan Mitra Bunda

DOI:

https://doi.org/10.59003/nhj.v5i5.1710

Keywords:

Mangkokan, antibactery, Bacillus cereus, Shigella dysentriae

Abstract

Mangkokan leaves (Polyscias scutellaria) are medicinal plants believed to treat the digestive tract, such as diarrhea, and abdominal pain, to use it by drinking a decoction of Mangkokan leaves as a traditional medicine. This study aims to determine the antibacterial activity of ethanol extract of Polyscias scutellaria leaves in inhibiting the growth of Bacillus cereus and Shigella dysentriae bacteria. The active compounds in Mangkokan leaves contain alkaloids, saponins, phenolics, and steroids. These ingredients can function as antibacterial. This study was carried out by maceration method using 96% ethanol solvent, and the viscous extract obtained tested to see the inhibition in Bacillus cereus and Shigella dysenteriae bacteria using the disc paper diffusion method with concentrations of 25%, 50%, 75%, and 100%. The positive control was chloramphenicol 30 μg/disk, and the negative control was DMSO 5% (Dimethylsulfoxide). The test results showed that Mangkokan leaf ethanol extract had antibacterial activity against Bacillus cereus bacteria with a (moderate) inhibitory response at a concentration of 100% of 7.96 mm. In comparison, Shigella dysentriae has an inhibitory response (moderate) at a concentration of 100% of 7.68 mm

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References

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12 (4), 564-582.

Devi, S., & Mulyani, T. (2017). Antibacterial Activity of Ethanol Extract Pacar Kuku Leaf (Lawsonia inermis Linn) in Pseudomonas aeruginosa. Journal Umbjm, 1 (1), 30-35.

Fitriyani, N., Ridhoni, M., & Rahmawati, Y. (2017). Uji aktivitas antibakteri ekstrak etanol daun mangkokan (Polyscias scutellaria) terhadap Staphylococcus aureus dan Escherichia coli. Jurnal Ilmiah Farmasi,

Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (3rd ed.). Chapman & Hall.

Helmin, S. M. (2021). Uji Aktivitas Antidiare Ekstrak Daun Mangkokan (Nothopanax scutellarium).

Himawan, H. C., Pramono, P., & Resti, D. A. (2017). Uji Farmakologis Ekstrak Kental Daun Meniran(Phyllanthus niruri Linn) Untuk Membantu Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus norvegicus strain Sprague-Dawley). Jurnal Farmamedika (Pharmamedica Journal), 2 (1), 30.

Kenedy, J, M., Suharyanisa, Situmorang, M., & Loi, A. (2022). Identifikasi Simplisia Dan Uji Aktivitas Aantibakteri Daun Ceremai (Phyllanthus acidus (L.) Skeels) Terhadap Bakteri Streptococcus pyogenes Dan Bakteri Salmonella typhi. Jurnal Tekesnos, 4 (1), 307-318

Nikaido, H. (2003). Molecular basis of bacterial outer membrane permeability revisited. Microbiology and Molecular Biology Reviews, 67 (4), 593–656.

Oktaviani, M., & Al Zahra, S. (2023). Review Artikel: Aktivitas Antibakteri Daun Mangkokan (Nothopanax scutellarium). Journal Syifa Sciences and Clinical Research, 5 (3), 489-498.

Putri, A. Y., Susanti, H., & Rahayu, R. (2020). Potensi antibakteri ekstrak etanol daun mangkokan (Polyscias scutellaria) terhadap bakteri Escherichia coli dan Salmonella typhi. Jurnal Kesehatan, 11 (2), 85-90.

Prasetya, W. (2010). Uji Aktivitas Antijamur Ekstrak Etanol Buah Ceremai (Phyllanthus acidus (L.) Skeels) Terhadap Candida albicans dan Trichophyton rubrum. Sukoharjo: Fakultas Farmasi, Universitas Muhammadiyah Surakarta.

Roslianizar, S., Priltius, N., Sitohang, R., & Rahmah. (2021). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Mangkokan (Polyscias scuterllaria (Bum.f.) Terhadap Pertumbuhan Bakteri Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa dan Escherichia coli. Jurnal Tekesnos, 2(3), 342-353.

Sabrina, A. P., Tania, E., Nurhalifah, N., Alvian, R., Veronita, S. C., Puji, S. I., & Nuryamah, S. (2022). Studi Fitokimia dan Farmakologi Daun Mangkokan (Nothopanax scutellarium). Jurnal Buana Farma, 2 (2), 33-39.

Sundaram, R., Dey, A., & Smitha, V. (2012). Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. Journal of Ethnopharmacology, 143 (2), 287-293.

Suhaera, S., Rachmayanti, A. S., & Aoliyaninda, N. (2022). Aktivitas Antibakteri Ekstrak Bronok (Acaudina molpadioides) terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Surya Medika, 8 (3), 133-137.

Yuliana Ayen, R., Rahmawati, M. (2017). Aktivitas Antibakteri Ekstrak Metanol Acalypha hispida Terhadap Bakteri Shigella flexneri dan Bacillus cereus IHB B 379. Jurnal TENGKAWANG, 10 (2), 123-129.

Yuliati. (2016). Uji Efektivitas Ekstrak Kunyit Sebagai Antibakteri Dalam Pertumbuhan Bacillus sp dan Shigella dysentriae Secara In Vitro. Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan, 10 (1), 1-7.