UJI KUERSETIN PADA EKSTRAK ETANOL LIDAH BUAYA (ALOE VERA BARBADENSIS) DENGAN METODE KROMATOGRAFI LAPIS TIPIS

Authors

  • Nadia Purnama Dewi Universitas Baiturrahmah
  • Ade Teti Vani
  • Dessy Abdullah
  • Meta Oktora
  • Fadilla Marisa

Keywords:

Aloe vera barbadensis; Quarcetin: Flavonoid; Thin layer chromatography;

Abstract

Aloe vera is a plant of the Liliaceae group. Aloe Barbadensis contains secondary metabolites that function as plant protection against pests and diseases. The grouping of secondary metabolites is known as phytochemical screening. Thin-layer chromatography (TLC) analysis is needed to isolate and identify the components of the secondary metabolites. Method: This is an experimental laboratory. The population was the Aloe Vera Barbadensis Millier species from Kalimantan, obtained from the Lubuk Minturun Agricultural Vocational School. The research process includes sample preparation, maceration extraction with 70% ethanol, flavonoid phytochemical test, and TLC test with kuersetin as a comparison. Result:, A phytochemical test, TLC test with three variations of eluent, namely Methanol: Aquadest (6:4), Butanol: Glacial Acetic Acid: Water (4:1:5). Conclusions: Aloe vera from Lubuk was positive for flavonoid.

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References

F. Yuza et al., “Efek Pemberian Ekstrak Lidah Buaya (Aloe Barbadensis Miller) pada Soket Gigi terhadap Kepadatan Serabut Kolagen Pasca Ekstraksi Gigi Marmut (Cavia Porcellus),” Maj. Kedokt. Gigi Indones., vol. 21, no. 2, pp. 127–135, Dec. 2014, doi: 10.22146/MAJKEDGIIND.8743.

M. H. Radha and N. P. Laxmipriya, “Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review,” J. Tradit. Complement. Med., vol. 5, no. 1, p. 21, Jan. 2015, doi: 10.1016/J.JTCME.2014.10.006.

R. Bista, A. Ghimire, and S. Subedi, “Phytochemicals and Antioxidant Activities of Aloe Vera (Aloe Barbadensis),” J. Nutr. Sci. Heal. Diet, vol. 1, no. 1, Jun. 2020, doi: 10.47890/JNSHD/2020/RBISTA/10243803.

M. Hęś, K. Dziedzic, D. Górecka, A. Jędrusek-Golińska, and E. Gujska, “Aloe vera (L.) Webb.: Natural Sources of Antioxidants – A Review,” 2019, doi: 10.1007/s11130-019-00747-5.

ade teti Vani, GEL ALOE VERA - Google Play Books, 1st ed. Indramayu: Adab, 2022.

S. Akbari, N. H. Abdurahman, R. M. Yunus, A. H. A. Alsaggaf, and N. Ahmed, “LC-QTOF-MS analysis of phenolics and saponins extracted from Aloe vera leaves via microwave technology in optimal condition,” South African J. Bot., vol. 139, pp. 362–373, Jul. 2021, doi: 10.1016/J.SAJB.2021.02.027.

nadia purnama Dewi, D. Abdullah, and ade teti Vani, “IDENTIFIKASI KUARSETIN DENGAN TEKNIK KLT DAN HPLC PADA ALOE VERA BARBADENSIS MILLER IDENTIFICATION OF QUARCETINE WITH TLC AND HPLC TECHNIQUES AT ALOE VERA BARBADENSIS MILLER | Request PDF,” Jurnal Kesehatan Medika Saintika, 2020. https://www.researchgate.net/publication/351095008_IDENTIFIKASI_KUARSETIN_DENGAN_TEKNIK_KLT_DAN_HPLC_PADA_ALOE_VERA_BARBADENSIS_MILLER_IDENTIFICATION_OF_QUARCETINE_WITH_TLC_AND_HPLC_TECHNIQUES_AT_ALOE_VERA_BARBADENSIS_MILLER (accessed Jan. 11, 2022).

M. Moniruzzaman, B. Rokeya, S. Ahmed, A. Bhowmik, M. I. Khalil, and S. H. Gan, “In vitro antioxidant effects of Aloe barbadensis Miller extracts and the potential role of these extracts as antidiabetic and antilipidemic agents on streptozotocin-induced type 2 diabetic model rats.,” Molecules, vol. 17, no. 11, pp. 12851–12867, Nov. 2012, doi: 10.3390/MOLECULES171112851.

K. J. S and R. N. Patil, “Ameliorative Effect of Aloe vera Leaf (Methanol) Extract on Blood Glucose Level and Histopathology of Pancreas in Alloxan Induced Diabetic Male Mice,” Print) Biosci. Discov., vol. 8, no. 3, pp. 528–532, 2017, Accessed: Mar. 23, 2022. [Online]. Available: http://biosciencediscovery.com.

B. Putri Bunga Anggreno Setiawan, A. Teti Vani, B. Yulhasfi Febrianto, V. Tri Septiana, and P. Bunga Anggreno Setiawan, “The Effectiveness of Using Aloe Vera Facial Soap and Aloe Gel on the Degree of Acne Vulgaris in Students of SMA Negeri 2 Bayang,” J. EduHealth, vol. 11, no. 1, pp. 39–47, Sep. 2020, doi: 10.54209/JURNALEDUHEALTH.V11I1.151.

A. Kasim, A. Asben, and A. Anwar, “Review: Optimalisasi Metode Maserasi Untuk Ekstraksi Tanin Rendemen Tinggi,” MENARA ilmu, vol. XIV, no. 02, pp. 38–41, 2020.

E. Susilowati and E. Surani, “HUBUNGAN POLA ASUH DENGAN PERKEMBANGAN MENTAL EMOSIONAL ANAK USIA PRA SEKOLAH DI KELURAHAN GEBANGSARI KECAMATAN GENUK KOTA SEMARANG,” J. Kesmas (Kesehatan Masyarakat) Khatulistiwa, vol. 7, no. 2, pp. 54–61, Jun. 2020, doi: 10.29406/JKMK.V7I2.2035.

N. Karima, “Identifikasi Senyawa Kuersetin Ekstrak Etil Asetat Daun Senggani (Melastoma malabathricum L.) Dengan Metode Kromatografi Lapis Tipis (KLT),” Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 2019. https://jurnal.untan.ac.id/index.php/jmfarmasi/article/view/37078 (accessed Mar. 24, 2022).

E. M. Sari, M. E. Sriyani, D. Isti, I. Halimah, and W. Nuraini, “Karakteristik Fisikokimia Senyawa Bertanda 99mTc-Kuersetin,” 2019. https://123dok.com/document/z3l6pkez-karakteristik-fisikokimia-senyawa-bertanda-mtc-kuersetin.html (accessed Mar. 24, 2022).

Y. A. Koirewoa, F. Fatimawali, and W. Wiyono, “ISOLASI DAN IDENTIFIKASI SENYAWA FLAVONOID DALAM DAUN BELUNTAS (Pluchea indica L.),” PHARMACON, vol. 1, no. 1, Aug. 2012, doi: 10.35799/PHA.1.2012.445.

D. G. Watson, Pharmaceutical analysis : a textbook for pharmacy students and pharmaceutical chemists. 2015.

S. Septiani and S. fatimah Muis, “AKTIVITAS ANTIOKSIDAN DAN KADAR ALOIN PADA LIDAH BUAYA (ALOE VERA CHINENSIS) | Septiani | JURNAL MEDIKA INDONESIA.” https://ejr.stikesmuhkudus.ac.id/index.php/JMI/article/view/1112 (accessed Jan. 11, 2022).

M. P. Quezada, C. Salinas, M. Gotteland, and L. Cardemil, “Acemannan and Fructans from Aloe vera (Aloe barbadensis Miller) Plants as Novel Prebiotics.,” J. Agric. Food Chem., vol. 65, no. 46, pp. 10029–10039, Nov. 2017, doi: 10.1021/ACS.JAFC.7B04100.

L. Wagner et al., “Herbal Medicine for Cough: A Systematic Review and Meta-Analysis,” Complement. Med. Res., vol. 22, no. 6, pp. 359–368, 2015, doi: 10.1159/000442111.

H. Wagner, S. (Sabine) Bladt, and E. M. (Eva M. Zgainski, “Plant drug analysis : a thin layer chromatography atlas,” p. 320, 2015.

Mulyanita, M. Djali, and I. siti Sitiasih, “Total Fenol, Flavonoid dan Aktivitas Antimikroba Ekstrak Limbah Kulit Lidah Buaya (ALOE CHINENSIS BAKER),” J. vokasi Kesehat., pp. 95–102, 2019.

E. Yunita and Z. Khodijah, “Pengaruh Konsentrasi Pelarut Etanol saat Maserasi terhadap Kadar Kuersetin Ekstrak Daun Asam Jawa (Tamarindus indica L.) secara Spektrofotometri UV-Vis,” Pharm. J. Farm. Indones. (Pharmaceutical J. Indones., vol. 17, no. 2, pp. 273–280, Dec. 2020, doi: 10.30595/PHARMACY.V17I2.6841.

R. A. Rahmaheni, “Uji Identifikasi Senyawa Kuersetin Dalam Ekstrak N-Heksan Daun Senggani (Melastoma malabathricum L.) Menggunakan Metode Kromatogafi Lapis Tipis. - CORE,” 2019. https://core.ac.uk/display/290043787 (accessed Mar. 25, 2022).

* Muthukumaran and E. Keerthika, “Total phenolic and flavonoid content of membrane processed Aloe vera extract: a comparative study,” Int. Food Res. J., vol. 25, no. 4, pp. 1450–1456, 2018.

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Published

2022-04-01

How to Cite

Nadia Purnama Dewi, Ade Teti Vani, Dessy Abdullah, Meta Oktora, & Fadilla Marisa. (2022). UJI KUERSETIN PADA EKSTRAK ETANOL LIDAH BUAYA (ALOE VERA BARBADENSIS) DENGAN METODE KROMATOGRAFI LAPIS TIPIS. Nusantara Hasana Journal, 1(11), 59–69. Retrieved from https://nusantarahasanajournal.com/index.php/nhj/article/view/287

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